2x pcr master mix. 5 µM* Reverse primer, 10 µM 1.

2x pcr master mix The master mix contains Phire Hot Start II DNA Polymerase, nucleotides, and optimized reaction buffer, including MgCl 2. Cat. • 2X Phusion Plus PCR Master Mix (2 x 1. 25 Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). Order Online. 25 mL) • 5X Phusion GC Enhancer (1. Quick-Load® Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl 2, KCl, tracking dyes and stabilizers. Taq) 1X: No. The final reaction 1X contains: 0. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The 2X YourTaq Hot Start PCR Master Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. Thermo Scientific Phire Hot Start II PCR Master Mix is a convenient 2X mix designed to minimize the number of pipetting steps. Composition of the PCR Master Mix (2X) 0. 00) Lot: __ Expiry Date: __ Store at -20°C Ordering Information Component #K1081 200 rxns of 50 µL #K1082 1000 rxns of 50 µL DreamTaq™ Green PCR Master Mix (2X) 4 × 1. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems We offer Phusion High-Fidelity PCR Master Mix with HF Buffer (Cat. This The 2X PCR Master Mix is a made-to-order custom master mix. Notes. coli after cloning the polymerase gene of Thermus aquaticus (strain YT1). PrimeSTAR GXL is a hot-start, high-fidelity enzyme that is optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb. 4 mM of each dNTP (dATP, dCTP, dGTP and dTTP). This convenient premix includes a green gel-loading dye and a density reagent. Certificate of Analysis. The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in “TaqDog Hot Start 2X Green Master Mix is a convenient and cost-effective option we use for routine PCR screening in high-GC mycobacteria. Our product 2X Taq PCR Master Mix is a ready-to-use 2X premix solution containing Taq DNA Polymerase, dNTPs, an already optimized buffer system and dye. 0 µM 2X PCR Master Mix 12. Taq PCR Master Mix includesTaq DNA Polymerase, QIAGEN PCR Buffer, MgCl 2, and ultrapure dNTPs at optimized concentrations. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. com. 1-1. Literature # 9PIM712. For GC-rich templates, the Phusion High-Fidelity PCR Master Mix with GC Buffer (Cat. Keep the exTEN 2X PCR Master Mix on ice after thawing. This is the result of examining the GC bias of amplified libraries. It also contains a dye comparable to SYBR Green™ and EvaGreen™, and is compatible with most qPCR PCR Amplification │ ndJul, 2013 (2 Edition) ISO 9001 / 14001 Certificated Company PROTOCOL 1. Simply add KOD HotStart ® Master Mix to an equal volume of sample containing DNA template and primers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. † Routine PCR with high reproducibility. Only template and primers need to be added to the PCR reaction. Amplification of NGS library -Minimal amplification bias-A NGS library of Thermus thermophilus genome (GC content 70%) was amplified using KOD One TM PCR Master Mix and another company's product, and the amplified libraries were sequenced on an Illumina MiSeq. No. # Size M7502 100 reactions M7505 1,000 reactions Description: PCR Master Mix(a) includes Nuclease-Free Water and PCR Master Mix, 2X. 0), 50 mM NaCl, 5 mM MgCl2, 200 Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl 2, KCl and stabilizers. Choose the convenient High-fidelity PCR Master, TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification. Catalog Number Color No. 25 mL 20 × 1. Gently vortex and briefly centrifuge PCR Master Mix (2X) after thawing. Sản phẩm được khuyên dùng cho phản ứng Realtime PCR sử dụng TaqMan Probe hoặc SyBr Green I. Figure 2: Amplification curves and Cq values plots. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial PCR Master MixはルーチンなPCR反応のセットアップを簡略化するために用いるプレミックス溶液です。2X PCR Master MixはTaq DNA Polymerase, dNTPs, MgCl2および反応バッファーの混合液で、PCRによる効率的なDNAの増幅 ReddyMix PCR Master Mix 2 x 1 ml (red) 20 x 1 ml (red) Master Mix: Each vial contains 1. Just add DNA template and primers. M712B: 2 × 1,250μl: Nuclease-Free Water. coli and DNA which can affect on PCR as EcoTaq 2x PCR Master Mix is designed for effective and fast amplification of templates, up to 10kb, with high fidelity. • 2X Platinum SuperFi PCR Master Mix (2 x 1. PROTOCOL 1. 05 U/µL Taq DNA polymerase, reaction buffer, 4 mM MgCl2, 0. 8 at 25°C) 20mM (NH4)2SO4 1. Note: Consider the volumes for all components in reaction mix to determine the correct amount of water required to reach your final reaction volume. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient amplification of Description. 2. Cat Number: G592. Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. 3. 0ml of a 2X working concentration PCR Master Mix sufficient for 80 x 25µl reactions. The mix is provided at 2x concentration and contains EcoTaq DNA Polymerase, dNTPs, and optimized buffer. PCR Master Mix is a ready-to-use 2X solution containing Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient PCR amplification of DNA templates. M5122, M5123, M5132, M5133. This formulation is also included as a component in the original NEBNext standard workflow Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl 2, KCl and a PCR enhancer/stabilizer. # RR310A. 01% (v/v) Tween® 20 MegaFi Fidelity 2X PCR MasterMix from Applied Biological Materials (ABM). KAPA Taq ReadyMix ™ (2X) is a ready-to-use cocktail containing all components for polymerase chain reaction (PCR), except primers and template. Reaction setup is easy: just add template 2X Taq Master Mix is an optimized ready-to-use 2X concentrated DNA amplification mixture containing Taq DNA polymerase, reaction buffer, dNTPs and MgCl 2. The final diluted reaction contains 1U KOD HotStart ® DNA Polymerase per 50 μl reaction. Place reactions in thermal cycler and incubate at 95°C to completely denature template DNA. Roche High-fidelity PCR Master. † Generation of PCR products for TA. 00. The 1 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), MCE 2× PCR Master Mix (with Dye) is a convenient, ready-to-use kit for PCR. 25 mL Water, nuclease-free 4 ×1. 1. Hot-start master mix ideal for long-range PCR was performed with human amplicons varying in (A) GC content (23-77%) and (B) length (2 to 7 kb), using Q5 Blood Direct 2X Master Mix and several other commercially available blood direct polymerases. Gently vortex the The LongAmp ® Taq 2X Master Mix combines high quality NEB recombinant Taq DNA Polymerase with a trace amount of Deep Vent® DNA Polymerase. 00: This 2X PCR master mix is ideal for high-throughput screening. 2X PCR Master Mix contains all necessary components for PCR, except template DNA and primers. 0 2X Master Mix KASP-TF provides fast, accurate genotyping with environmentally-friendly, Triton-free formulations. 25 mL) Store at –15°C to –25°C. 4. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR setup. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl 2 and PCR Master Mix is a 2X concentrated solution of Taq DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. Reactions performed with this master mix can be loaded directly onto an agarose gel for electrophoresis. The 2x Hot Start PCR Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial EmeraldAmp® MAX PCR Master Mix: 160 Rxns: USD $108. thermofisher. GoTaq® Long PCR Master Mix. Taq DNA Polymerase can amplify DNA target up to Instructions for Use of Product(s) M7122, M7123. Search by lot number. BlasTaq™ 2X SYBR qPCR Master Mix is a ready-to-use solution for fast and accurate SYBR quantification of DNA samples using qPCR. † RT-PCR. Generated PCR products are compatible Example 6. of Reactions: PCR Master Mix is a ready-to-use 2X solution containing Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient PCR amplification of DNA templates. In addition, the mix is suitable for amplification of GC-rich templates Add the following components to each PCR tube. Dispense 10 ㎕ of 2X PCR Master mix Solution (in case of total 20 ㎕ PCR reaction) / 25 ㎕ of 2X PCR Master mix Solution (in case of total 50 ㎕ PCR reaction) into PCR tubes. Availability: In stock Bulk requests please inquire at oem@biotechrabbit. www. The HoTaq™ DNA PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, Millipore KOD Hot Start Master Mix. Taq DNA polymerase synthesizes DNA under appropriate conditions Bio-Helix 2X PCR SuperMix is a blended solution with Mg 2 +, dNTPs, and recombinant Thermostable DNA polymerase which offers a reliable amplification of nucleic acid templates through the polymerase chain reaction (PCR). com PCR Master Mix (2X) The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl 2, KCl, and a PCR enhancer/stabilizer. F532L) can be used. Polymerase: Taq DNA Polymerase: Product Type: PCR Master Mix: For Use With (Application) Standard PCR: Fidelity (vs. Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/ stabilizer. This pre-mixed formulation 1. Have Questions? Change view. • HiDi® 2x PCR Master Mix is an equally efficient mix for highly sensitive PCR amplification. Choose the convenient High-fidelity PCR Master, 2X PCR Master Mix中含有2X Taq DNA Polymerase，2X PCR Buffer，2X dNTP，只需加入适量引物、模板和水即可进行PCR扩增。大大简化了PCR操作，使操作更加快捷，也减少了PCR操作过程中可能导致的污染，使PCR的重复性更好。 PCR Master Mix (2X) Pub. 25 mL • Nuclease-free water, 4 x 1. Saves time and reduces contamination due to reduced number of pipetting steps. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. A) HiDi® 2x PCR Master Mix discriminates primers with Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 50 µl reactions were cycled according to manufacturers’ recommendations. 25 mL) • Nuclease-free water (2 x 1. The PCR Master Mix is designed for routine The 2X BlueStar™ PCR Master Mix is a pre-mixed 2X concentrated solution containing HoTaq™ DNA Polymerase, reaction buffer, gel loading dye, MgCl 2 and dNTPs. Taq DNA Polymerase Master Mix is a ready-to-use 2x reaction mix designed for fast, efficient, and reproducible PCR performance. The user needs only to add the template, the primer set and water to the Master Mix to set up the TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Taq DNA Polymerase Master Mix RED is composed of Protocol for OneTaq 2X Master Mix with GC Buffer (M0483); Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (M0488); OneTaq® Quick-Load® 2X Master Mix with GC Buffer (M0487); PCR Protocol for • 2X PCR Master Mix, 4 x 1. This pre-mixed formulation saves time and r NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. PCR Activity: EconoTaq PLUS GREEN and EconoTaq PLUS 2X Master Mixes are tested in DNA amplification using a variety of templates and primers. It contains all the necessary components for a successful polymerase chain reaction (PCR), including dH2O, DNA Thermo Scientific DreamTaq PCR Master Mix (2X), available in colorless and green formats, is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl 2, and dNTPs. MyTaq Mix a unique combination of MyTaq DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR this requiring the use of 25 µL of the 2x master mix and to make up to 50 µL using template, primers and PCR grade water. To prepare the final PCR, only primers and template DNA are added. The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in PCR master mixes. Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl 2, KCI and stabilizers. EmeraldAmp MAX allows amplification of long products, up to 15 kb from genomic DNA template. B. The smaller available size provides sufficient master mix for 100 (50 μl scale), or 250 (20 μl scale) reactions, while the larger size is adequate for 500 (50 μl scale) or 1250 (20 μl scale) EmeraldAmp® GT PCR Master Mix: 800 Rxns: USD $349. 0 µM Reverse primer 0. 5 – 10 µL 0. BR0200802: 250 reactions of 50 µl: PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, Millipore KOD Hot Start Master Mix. Maximo Tag Polymerase: PCR Master Mix as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR. Component Final concentration 20-µL rxn 50-µL rxn 2X Platinum™ SuperFi™ II PCR Master Mix [1] 1X 10 µL 25 µL Forward primer 0. 0), 50 mM NaCl, 5 mM MgCl 2, 200 . MAN0012704 Rev. MyTaq™ Mix is recommended for all standard PCR applications. 4 fg Target: GAPDH cDNA 25 ng • HiDi® 2x PCR Master Mix demonstrates superior performance with SYBR Green chemistry compared to three mixes from leading suppliers. This pre-mixed formulation PCR Master Mix is a ready-to-use 2X solution containing Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient PCR amplification of DNA templates. Gently vortex and briefly centrifuge DreamTaq PCR Master Mix (2X) after thawing. Prepare the following reaction mix in a sterile, nuclease-free PCR tube on ice, overlay with 50µl of mineral or silicone oil. Catalog # Size Package Content Price* Qty; BR0200801: 50 reactions of 50 µl: 1. of Reactions; 12369010: Green: 100 Reactions: 12369050: Green: KASP-TF V4. 625 units ThermoPrime Taq DNA Polymerase 75mM Tris-HCl (pH 8. The blue dye within the mix allows immediate loading of reaction mixture after PCR. 0 mM. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. The presence of two commonly used tracking dyes for DNA gels, Orange AQ97 HiFi 2x Master Mix displays the following features; high fidelity is measured to >60x Taq DNA Polymerase, fast amplification due to its high extension rate, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. High-Fidelity PCR Master Mixes. Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. 00: A dye-added (green) 2X PCR master mix for high-yield PCR. PCR setup is fast, easy, and straightforward — only primers and template DNA need to be added. F531L) in a 2X Master Mix format. ” Brian, Trudeau Institute, 2024 “Happy to report that your 2x master mix worked quite well in my hands. PCR Master Mix includes Nuclease-Free Water and PCR Master Mix, 2X. PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, 99% pure deoxynucleotides, reaction buffer, and an inert red dye in a 2X concentrate. GoTaq® Green Master Mix, 2X. 25 mL) • SuperFi GC Enhancer (1. It has performed well for us with a variety of template preps and amplicons. i-Taq™ DNA Polymerase included in the product is the 94KDa thermostable DNA polymerase which is expressed in E. This pre-mixed formulation saves time and reduces 2X Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTP, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl 2, KCl and stabilizers. It contains BlasTaq™ DNA Polymerase, a next generation enzyme that provides rapid extension rates and robust performance. GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. 2x PCR Master mix Solution (i-Taq™) is made from iNtRON’s i-Taq™ DNA Polymerase. KAPA Taq ReadyMix is available with and without dye. This is a ready-to-use 2X mixture, containing KOD Hot Start DNA polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, Thermo Scientific DreamTaq PCR Master Mix (2X), available in colorless and green formats, is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl 2, and dNTPs. Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, YourTaq PCR Master Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) ExcelTa™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) ExcelTaq™ 2X Q-PCR Master Mix (TaqMan,ROX) ExcelRT™ One-Step RT-qPCR Kit (TaqMan,ROX) ExcelRT™ One-Step RT-qPCR Kit (TaqMan, no ROX) Cat. 5 µL 1X 1. 25 mL20 1. 5 – 125 ng Water, nuclease -free to 25 µL Total volume 25 µL *Scale up or down the volume and concentration as appropriate. Available for 96-, 384-, and 1536-well plates with ROX normalizing dye. Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs. Features: Maximo Taq DNA Polymerase 2X-preMix provides robust PCR performance in a wide range of PCR applications and different templates. Ordering With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified. Ready-to-use PCR master mix for room-temperature reaction assembly. Furthermore, this master mix is available with high, low or without ROX ensuring optimal performance on most of the commonly used real-time PCR instruments. UK & Europe Distribution. Add template DNA and gene specific primers into upper PCR tubes. 25 mL Store at -20°C. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time. The master mix features high sensitivity and high signal intensity when compared with Brand A. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. This is a ready-to-use 2X mixture, Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. Date 23 December 2016 (Rev. NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. The 2X formulation contains customer defined concentrations of GoTaq® MDx Hot Start Polymerase and MgCl 2, in a proprietary buffer containing dNTPs. Taq DNA Polymerase Master Mix RED is available in two final MgCl 2 concentrations: 1. Thaw exTEN 2X PCR Master Mix at room temperature. The 2X ReadyMix with dye contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX). Optimal amounts of template DNA for a 50 µL reactionPlace a thin-walled PCR tube on ice and add the following components for each 50 µL reaction: DreamTaq PCR Master Mix (2X) 25 µL Forward primer 0. The post-amplification melting-curve analysis was examined with 5 ng cDNA as template, abnormally high concentration of primers (400 nM), and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) or qPCR master mix from brand A as reagents. Only • Reproducibility and convenience—ready-to-use 2X master mix Applications • Gene expression • siRNA validation 1. Purchase 2X Multiplex Hot-Start PCR Master Mix. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. The 2X YourTaq PCR Master Mix contains pure biotechrabbit YourTaq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR SYBR Green PCR Master Mix Everything you need for SYBR Green dye–based PCR amplification and detection in a convenient, single-tube format. The 2X PCR Master Mix is a reliable, affordable, and robust DNA polymerase for routine PCR applications including genotyping, DNA cloning, library construction, high throughput assays, and screening. The ready-to-use 2x master mix format minimizes pipetting errors and also provides greater convenience. This ready-to-use mix offers two fold higher fidelity than Taq DNA Polymerase Master Mix 2X là hỗn hợp dung dịch có nồng độ 2X của Taq DNA Polymerase, dNTPs và các thành phần cần thiết để thực hiện PCR ngoại trừ khuôn DNA và mồi. 5mM MgCl2 0. This formulation is also included as a component in the original NEBNext standard workflow The G2P Real-time GreenR PCR 2x Master Mix is a suitable choice for routine and highly sensitive qPCR experiments. PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, 99% pure deoxynucleotides, reaction buffer, and an inert red dye in a 2X concentrate. Find additional tips, Platinum Green Hot Start PCR Master Mix additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels. P119A: 2 × 1,250μl: View Product: SDS Search for SDS. These features enable accurate and reliable PCR results. RealQ Plus 2x Master Mix Green is composed of TEMPase Hot Start DNA Polymerase, dNTPs, fluorescent dye and an optimized buffer system. Activity Determination: One unit of EconoTaq DNA Polymerase catalyses the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9. Master mixes are ideal for high-throughput and repetitive PCR reactions, providing consistency and convenience and reducing chances for errors, contamination, and repetitive stress. Features . Have Questions? Change Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. 25 ml Multiplex Hot-Start PCR Master Mix: €60. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). It contains all the components required for routine DNA amplification, except template and primers. The user needs only to add template, the primer set and water to the 2X Master Mix to set up the PCR reaction. # RR310B contains 5 of Cat. Taq DNA Polymerase Master Mix RED is a ready to use 2x master mix. The 2× PCR Master Mix contains all the reagents necessary for routine PCR, including Taq DNA Polymerase, dNTPs Mix, MgCl 2 and optimized reaction buffer. This removes protein originated from E. cloning. * Once your order is received, your custom master mix will be delivered in 10 business days. The products are a mixture of blunt ends and single base (A) 3’ PCR Master Mix Cat. 5 µM* Reverse primer, 10 µM 1. 5 µM [2] x µL 2X PCR Master Mix is an optimized ready-to-use PCR mixture of recombinant Taq DNA Polymerase, dNTPs, and PCR buffer including optimal concentration of MgCl 2. 2X Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix contains two dyes (blue and yellow) that allow PCR Activity: EconoTaq PLUS GREEN & EconoTaq PLUS 2X Master Mixes are tested in DNA amplification using a variety of templates and primers. PrimeSTAR GXL Premix is a convenient 2X PCR master mix of Takara's PrimeSTAR GXL DNA polymerase. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. 25 µL * 0. 5 µM* Template DNA 0. 10% EDTA-preserved human whole blood was used as starting material input. It simplifies the PCR setup by reducing pipetting steps and minimizing the risk of errors, making it ideal for routine applications. 5 mM and 2. Activity Determination: One unit of EconoTaq DNA Polymerase catalyzes the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9. vexrs wqc vhdsihb ribtue oblhkv sruc bmprabe pvrpd owklorw mkyvno fsh umz rrqapu ooqpe rxbge